Invitrogen

BCAS2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (11) BCAS2 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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BCAS2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of BCAS2 in U-2 OS cells using a BCAS2 polyclonal antibody (Product # PA5-84771). The analysis shows localization to nuclear speckles & centrosome.

BCAS2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of BCAS2 in human thyroid gland and pancreas tissues using a BCAS2 polyclonal antibody (Product # PA5-84771). Corresponding RNA-seq data are presented for the same tissues.

BCAS2 Antibody in Western Blot (WB) — Western blot analysis of BCAS2 by a BCAS2 polyclonal antibody (Product # PA5-84771). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG.

BCAS2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of BCAS2 demonstrates antibody specificity. Immunohistochemical analysis of BCAS2 using anti-BCAS2 Polyclonal Antibody (Product #PA5-84771), shows significant staining of BCAS2 in thyroid gland and shows minimal or weak staining in pancreas tissues. The relative expression levels of BCAS2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-84771

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human BCAS2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2791921

Product Specific Information

Immunogen sequence: MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL S

Target Information

cDNA encoding BCAS2 has been isolated. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells. BCAS2 is ubiquitously expressed in normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Pre-mRNA-splicing factor SPF27; spliceosome associated protein, amplified in breast cancer; Spliceosome-associated protein SPF 27

Gene Aliases: BCAS2; DAM1; Snt309; SPF27

UniProt ID: (Human) O75934

Entrez Gene ID: (Human) 10286

Molecular Function: RNA splicing factor