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BCL2L13 Polyclonal Antibody

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BCL2L13 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using BCL2L13 antibody 16612-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

BCL2L13 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using (Product # 16612-1-AP) (BCL2L13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BCL2L13 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

BCL2L13 Antibody in Immunoprecipitation (IP) — IP result of anti-BCL2L13 (IP:16612-1-AP, 3ug; Detection:16612-1-AP 1:100) with mouse heart tissue lysate 8000ug.

Product Details

16612-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BCL2L13 Fusion Protein Ag9841 (1-300 aa encoded by BC003032) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MLLELTRRG QEPLSALLQF GVTYLEDYSA EYIIQQGGWG TVFSLESEEE EYPGITAEDS NDIYILPSDN SGQVSPPESP TVTTSWQSES LPVSLSASQS WHTESLPVSL GPESWQQIAM DPEEVKSLDS NGAGEKSENN SSNSDIVHVE KEEVPEGMEE AAVASVVLPA RELQEALPEA PAPLLPHITA TSLLGTREPD TEVITVEKSS PATSLFVELD EEEVKAATTE PTEVEEVVPA LEPTETLLSE KEINAREESL VEELSPASEK KPVPPSEGKS RLSPAGEMKP MPLSEGKSIL L (1-300 aa encoded by BC003032)

Target Information

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A novel role for Bcl2l13 in promoting beige adipocyte biogenesis.

Biochemical and biophysical research communications

Ju L,Chen S,Alimujiang M,Bai N,Yan H,Fang Q,Han J,Ma X,Yang Y,Jia W

Fri Nov 30 00:00:00 EST 2018

Degradation of altered mitochondria by autophagy is impaired in Lafora disease.

The FEBS journal

Lahuerta M,Aguado C,Sánchez-Martín P,Sanz P,Knecht E

Fri Jun 01 00:00:00 EDT 2018

Low-dose rapamycin extends lifespan in a mouse model of mtDNA depletion syndrome.

Human molecular genetics

Siegmund SE,Yang H,Sharma R,Javors M,Skinner O,Mootha V,Hirano M,Schon EA

Fri Dec 01 00:00:00 EST 2017

Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.

Nature communications

Murakawa T,Yamaguchi O,Hashimoto A,Hikoso S,Takeda T,Oka T,Yasui H,Ueda H,Akazawa Y,Nakayama H,Taneike M,Misaka T,Omiya S,Shah AM,Yamamoto A,Nishida K,Ohsumi Y,Okamoto K,Sakata Y,Otsu K

Mon Jul 06 00:00:00 EDT 2015

Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.

Proceedings of the National Academy of Sciences of the United States of America

Jensen SA,Calvert AE,Volpert G,Kouri FM,Hurley LA,Luciano JP,Wu Y,Chalastanis A,Futerman AH,Stegh AH

Tue Apr 15 00:00:00 EDT 2014

Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.

Proceedings of the National Academy of Sciences of the United States of America

Jensen SA,Calvert AE,Volpert G,Kouri FM,Hurley LA,Luciano JP,Wu Y,Chalastanis A,Futerman AH,Stegh AH

Tue Apr 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Bcl-2-like protein 13; Bcl-rambo; Bcl2-L-13; BCL2-like 13 (apoptosis facilitator); mitochondria located 1 homolog; Protein Mil1

Gene Aliases: BCL-RAMBO; Bcl2-L-13; BCL2L13; CD003; E430016C20Rik; Mil-1; MIL1

UniProt ID: (Human) Q9BXK5, (Mouse) P59017

Entrez Gene ID: (Human) 23786, (Mouse) 94044, (Rat) 312682

Function(s)

apoptotic process regulation of apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process

Process(es)

protein binding cysteine-type endopeptidase activator activity involved in apoptotic process molecular_function

BCL2L13 Polyclonal Antibody

Product Details

16612-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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