Immunofluorescent analysis of BCL7C showing staining in the cytoplasm and nucleus of Jurkat cells. Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a BCL7C polyclonal antibody (Product # PA5-30308) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 215 of Human BCL7C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30308 targets BCL7C in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30308 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 215 of Human BCL7C.
This gene is identified by the similarity of its product to the N-terminal region of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. The function of this gene has not yet been determined.
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