Proteintech

BHLHE22 Polyclonal Antibody

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BHLHE22 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SH-SY5Y cells using 25622-1-AP (BHLHE22 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

BHLHE22 Antibody in Western Blot (WB) — SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25622-1-AP ( BHLHE22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

25622-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Product Specifications

Species Reactivity

Human, Mouse

Published species

Goat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BHLHE22 Fusion Protein Ag20966 (1-225 aa encoded by BC156671)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MERGMHLGA AAAGEDDLFL HKSLSASTSK RLEAAFRSTP PGMDLSLAPP PRERPASSSS SPLGCFEPAD PEGAGLLLPP PGGGGGGSAG SGGGGGGGVG VPGLLVGSAG VGGDPSLSSL PAGAALCLKY GESASRGSVA ESSGGEQSPD DDSDGRCELV LRAGVADPRA SPGAGGGGAK AAEGCSNAHL HGGASVPPGG LGGGGGGGSS SGSSGGGGGS GSGSGG (1-225 aa encoded by B C156671)

Target Information

BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deubiquitinase OTUD3 regulates metabolism homeostasis in response to nutritional stresses.

Cell metabolism

Zhou N,Qi H,Liu J,Zhang G,Liu J,Liu N,Zhu M,Zhao X,Song C,Zhou Z,Gong J,Li R,Bai X,Jin Y,Song Y,Yin Y

Tue Jul 05 00:00:00 EDT 2022

Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary.

PloS one

An X,Song Y,Hou J,Zhang Y,Chen K,Ma H,Zhao X,Li G,Gao K,Wang S,Cao B,Bai Y

Mon Sep 25 00:00:00 EDT 2017

Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary.

PloS one

An X,Song Y,Hou J,Zhang Y,Chen K,Ma H,Zhao X,Li G,Gao K,Wang S,Cao B,Bai Y

Mon Sep 25 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: basic helix-loop-helix (bHLH) gene, class B, beta3; basic helix-loop-helix domain containing, class B, 5; basic helix-loop-helix domain containing, class B5; basic helix-loop-helix family, member e22; bHLHb5; bHLHe22; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; Protein BETA3; trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein

Gene Aliases: Beta3; BHLHB5; BHLHE22; CAGL85; TNRC20

UniProt ID: (Human) Q8NFJ8, (Mouse) Q8C6A8

Entrez Gene ID: (Human) 27319, (Mouse) 59058

Molecular Function: basic helix-loop-helix transcription factor