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BID Polyclonal Antibody

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BID Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 10988-1-AP (BID antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

BID Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10988-1-AP (BID antibody) at dilution of 1:100 (under 10x lens).

BID Antibody in Western Blot (WB) — Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 10988-1-AP ( BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

BID Antibody in Immunoprecipitation (IP) — IP result of anti-BID (IP:10988-1-AP, 4ug; Detection:10988-1-AP 1:500) with HeLa cells lysate 1280 ug.

Product Details

10988-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 22 publications 22 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BID Fusion Protein Ag1445 (1-195 aa encoded by BC009197) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MDCEVNNGS SLRDECITNL LVFGFLQSCS DNSFRRELDA LGHELPVLAP QWEGYDELQT DGNRSSHSRL GRIEADSESQ EDIIRNIARH LAQVGDSMDR SIPPGLVNGL ALQLRNTSRS EEDRNRDLAT ALEQLLQAYP RDMEKEKTML VLALLLAKKV ASHTPSLLRD VFHTTVNFIN QNLRTYVRSL ARNGMD (1-195 aa encoded by B C009197)

Target Information

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Santamarine Inhibits NF-кB and STAT3 Activation and Induces Apoptosis in HepG2 Liver Cancer Cells via Oxidative Stress.

Journal of Cancer

Mehmood T,Maryam A,Tian X,Khan M,Ma T

Wed Mar 30 00:00:00 EDT 2022

P53/PUMA are potential targets that mediate the protection of brain-derived neurotrophic factor (BDNF)/TrkB from etoposide-induced cell death in neuroblastoma (NB).

Apoptosis : an international journal on programmed cell death

Hua Z,Zhan Y,Zhang S,Dong Y,Jiang M,Tan F,Liu Z,Thiele CJ,Li Z

Wed Aug 01 00:00:00 EDT 2018

T-2 toxin-induced toxicity in neuroblastoma-2a cells involves the generation of reactive oxygen, mitochondrial dysfunction and inhibition of Nrf2/HO-1 pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Zhang X,Wang Y,Velkov T,Tang S,Dai C

Sun Apr 01 00:00:00 EDT 2018

ML-7 amplifies the quinocetone-induced cell death through akt and MAPK-mediated apoptosis on HepG2 cell line.

Toxicology mechanisms and methods

Zhou Y,Zhang S,Deng S,Dai C,Tang S,Yang X,Li D,Zhao K,Xiao X

Wed Dec 14 00:00:00 EST 2016

Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.

Oncology letters

Xu Y,Wu D,Fan Y,Li P,Du H,Shi J,Wang D,Zhou X

Sat Oct 01 00:00:00 EDT 2016

Dioscin Induces Apoptosis in Human Cervical Carcinoma HeLa and SiHa Cells through ROS-Mediated DNA Damage and the Mitochondrial Signaling Pathway.

Molecules (Basel, Switzerland)

Zhao X,Tao X,Xu L,Yin L,Qi Y,Xu Y,Han X,Peng J

Sat Jun 04 00:00:00 EDT 2016

FOXO3-mediated up-regulation of Bim contributes to rhein-induced cancer cell apoptosis.

Apoptosis : an international journal on programmed cell death

Wang J,Liu S,Yin Y,Li M,Wang B,Yang L,Jiang Y

Sun Mar 01 00:00:00 EST 2015

Synergistic antitumor activity of withaferin A combined with oxaliplatin triggers reactive oxygen species-mediated inactivation of the PI3K/AKT pathway in human pancreatic cancer cells.

Cancer letters

Li X,Zhu F,Jiang J,Sun C,Wang X,Shen M,Tian R,Shi C,Xu M,Peng F,Guo X,Wang M,Qin R

Sun Feb 01 00:00:00 EST 2015

ABT-263 enhances sorafenib-induced apoptosis associated with Akt activity and the expression of Bax and p21((CIP1/WAF1)) in human cancer cells.

British journal of pharmacology

Li J,Chen Y,Wan J,Liu X,Yu C,Li W

Tue Jul 01 00:00:00 EDT 2014

mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma.

International journal of clinical and experimental medicine

Zhao L,Teng B,Wen L,Feng Q,Wang H,Li N,Wang Y,Liang Z

Thu Mar 06 00:00:00 EST 2014

TNFR1/TNF-α and mitochondria interrelated signaling pathway mediates quinocetone-induced apoptosis in HepG2 cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Zhang C,Wang C,Tang S,Sun Y,Zhao D,Zhang S,Deng S,Zhou Y,Xiao X

Sun Dec 01 00:00:00 EST 2013

Synergistic anti-glioma effect of Hydroxygenkwanin and Apigenin in vitro.

Chemico-biological interactions

Wang Y,Xu YS,Yin LH,Xu LN,Peng JY,Zhou H,Kang W

Mon Nov 25 00:00:00 EST 2013

Anti-cancer effects of dioscin on three kinds of human lung cancer cell lines through inducing DNA damage and activating mitochondrial signal pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Wei Y,Xu Y,Han X,Qi Y,Xu L,Xu Y,Yin L,Sun H,Liu K,Peng J

Sun Sep 01 00:00:00 EDT 2013

Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways.

Journal of applied toxicology : JAT

Hu M,Xu L,Yin L,Qi Y,Li H,Xu Y,Han X,Peng J,Wan X

Thu Aug 01 00:00:00 EDT 2013

Synergistic antitumour activity of sorafenib in combination with tetrandrine is mediated by reactive oxygen species (ROS)/Akt signaling.

British journal of cancer

Wan J,Liu T,Mei L,Li J,Gong K,Yu C,Li W

Tue Jul 23 00:00:00 EDT 2013

Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.

PloS one

Yu G,Jiang L,Xu Y,Guo H,Liu H,Zhang Y,Yang H,Yuan C,Ma J

Tue May 07 00:00:00 EDT 2013

Total flavonoids from Rosa Laevigata Michx fruit attenuates hydrogen peroxide induced injury in human umbilical vein endothelial cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Jia Y,Ji L,Zhang S,Xu L,Yin L,Li L,Zhao Y,Peng J

Sat Sep 01 00:00:00 EDT 2012

Alantolactone induces activation of apoptosis in human hepatoma cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Lei JC,Yu JQ,Yin Y,Liu YW,Zou GL

Sat Sep 01 00:00:00 EDT 2012

CS055 (Chidamide/HBI-8000), a novel histone deacetylase inhibitor, induces G1 arrest, ROS-dependent apoptosis and differentiation in human leukaemia cells.

The Biochemical journal

Gong K,Xie J,Yi H,Li W

Tue May 01 00:00:00 EDT 2012

Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma.

Free radical biology & medicine

Gong K,Li W

Thu Dec 15 00:00:00 EST 2011

Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.

International journal of cancer

Liu C,Gong K,Mao X,Li W

Thu Sep 15 00:00:00 EDT 2011

alpha-Bisabolol induces dose- and time-dependent apoptosis in HepG2 cells via a Fas- and mitochondrial-related pathway, involves p53 and NFkappaB.

Biochemical pharmacology

Chen W,Hou J,Yin Y,Jang J,Zheng Z,Fan H,Zou G

Thu Jul 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID

Gene Aliases: BID; FP497

UniProt ID: (Human) P55957

Entrez Gene ID: (Human) 637

Function(s)