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Invitrogen
BID Polyclonal Antibody
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FIGURE: 1 / 3
BID Antibody (PA1-26454) in WB
Western blot was performed using Anti-BID Polyclonal Antibody (Product # PA1-26454) and a 20 kDa band corresponding to BID was observed across cell lines and decreased upon Staurosporine and also with Actinomycin D along with TNF Alpha treatment. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), A-431 treated with Staurosporine (1uM for 4 hr) (Lane 2), A-431 treated with Staurosporine (1uM for 8 hr) (Lane 3) and A-431 treated with Actinomycin along with TNF Alpha (5ug/ml and 10ng/ml for 5 hr) (Lane 4), MCF-7 (Lane 5), Hep G2 (Lane 6), ... View More
Product Details
PA1-26454
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the region surrounding amino acid 37 of human Bid.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
PA1-26454 detects Bid from human samples.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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