Invitrogen

BID Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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BID Antibody in Western Blot (WB) — Western blot was performed using Anti-BID Polyclonal Antibody (Product # PA5-17855) and a 20 kDa band corresponding to BID was observed across cell line and tissue extracts tested and decreased upon Staurosporine treatment. The expression of BID is reported to be low in Mouse Brain compared to Mouse Spleen and Mouse Thymus. Fig.a shows whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), NIH/3T3 treated with Staurosporine (1uM for 4 hr) and Fig.b shows tissue extracts of Mouse Spleen (Lane 1), Mouse Thymus (Lane 2) and Mouse Brain (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

BID Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of BID was observed in Mouse Spleen and Mouse Thymus in comparison to Mouse Brain using Anti-BID Polyclonal Antibody (Product # PA5-17855) in Western Blot. {RE}

Product Details

PA5-17855

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues surrounding the cleavage site of mouse BID
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_10978504

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apoptic d; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; BID; HGNC:1050; MGC15319; MGC42355; OTTHUMP00000196197; p11 BID; p13 BID; p15 BID; p22 BID

Gene Aliases: 2700049M22Rik; AI875481; AU022477; Bid

UniProt ID: (Mouse) P70444

Entrez Gene ID: (Mouse) 12122

Function(s)