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Invitrogen
BID Polyclonal Antibody
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FIGURE: 1 / 3
BID Antibody (PA5-17855) in WB
Western blot was performed using Anti-BID Polyclonal Antibody (Product # PA5-17855) and a 20 kDa band corresponding to BID was observed across cell line and tissue extracts tested and decreased upon Staurosporine treatment. The expression of BID is reported to be low in Mouse Brain compared to Mouse Spleen and Mouse Thymus. Fig.a shows whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), NIH/3T3 treated with Staurosporine (1uM for 4 hr) and Fig.b shows tissue extracts of Mouse Spleen (Lane 1), Mouse Thymus (Lane 2) and Mouse Brain (Lane... View More
Product Details
PA5-17855
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding the cleavage site of mouse BID
Conjugate
Form
Liquid
Concentration
240 µg/mL
Amount
24 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptic d; BH3-interacting domain death agonist; BID; HGNC:1050; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID
Gene Aliases: 2700049M22Rik; AI875481; AU022477; Bid
UniProt ID: (Mouse) P70444
Entrez Gene ID: (Mouse) 12122
release of cytochrome c from mitochondria
protein targeting to mitochondrion
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
apoptotic mitochondrial changes
regulation of protein oligomerization
positive regulation of protein oligomerization
positive regulation of protein homooligomerization
regulation of cell proliferation
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
establishment of protein localization to membrane
positive regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
mitochondrial outer membrane permeabilization
regulation of mitochondrial membrane permeability involved in apoptotic process
regulation of G1/S transition of mitotic cell cycle
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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