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Invitrogen
BID Polyclonal Antibody
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FIGURE: 1 / 4
BID Antibody (PA5-85135) in WB
Western blot analysis of BID in non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg). Samples were incubated with BID polyclonal antibody (Product # PA5-85135) using a dilution of 1:5,000 followed by HRP-conjugated anti-rabbit IgG. Separated by 12% SDS-PAGE.
Product Details
PA5-85135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Bid. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Positive Control: A431, H1299, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID
Gene Aliases: BID; FP497
UniProt ID: (Human) P55957
Entrez Gene ID: (Human) 637
release of cytochrome c from mitochondria
protein targeting to mitochondrion
activation of cysteine-type endopeptidase activity involved in apoptotic process
brain development
extrinsic apoptotic signaling pathway via death domain receptors
apoptotic mitochondrial changes
response to estradiol
positive regulation of protein oligomerization
positive regulation of protein homooligomerization
glial cell apoptotic process
regulation of cell proliferation
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
neuron apoptotic process
establishment of protein localization to membrane
positive regulation of release of cytochrome c from mitochondria
hepatocyte apoptotic process
mitochondrial outer membrane permeabilization
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
regulation of G1/S transition of mitotic cell cycle
positive regulation of extrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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