|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1235 and 1417 of Human BLM|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA5-27384 targets BLM in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27384 immunogen is recombinant fragment corresponding to a region within amino acids 1235 and 1417 of Human BLM.
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of alternative lengthening of telomere markers in BRCA1 defective cells.
PA5-27384 was used in immunocytochemistry to analyze telomere markers and alternative lengthening in BRCA1 defective cells
|Kargaran PK,Yasaei H,Anjomani-Virmouni S,Mangiapane G,Slijepcevic P||Genes, chromosomes and cancer (55:864)||2016|