Invitrogen

BLMH Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (10) BLMH antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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BLMH Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of BLMH in human skin and liver tissues using a BLMH polyclonal antibody (Product # PA5-83468). Corresponding RNA-seq data are presented for the same tissues.

BLMH Antibody in Western Blot (WB) — Western blot analysis of BLMH by a BLMH polyclonal antibody (Product # PA5-83468). Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).

BLMH Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of BLMH demonstrates antibody specificity. Immunohistochemical analysis of BLMH using anti-BLMH Polyclonal Antibody (Product #PA5-83468), shows significant staining of BLMH in skin and shows minimal or weak staining in liver tissues. The relative expression levels of BLMH within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-83468

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:5,000-1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human BLMH View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2790623

Product Specific Information

Immunogen sequence: RKEPEDGRLV QFLLMNPAND GGQWDMLVNI VEKYGVIPKK CFPESYTTEA TRRMNDILNH KMREFCIRLR NLVHSGATKG EISATQDVMM EEIFRVVCIC LGNP

Target Information

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.