Immunofluorescent analysis of BMI-1 in the nucleus of methanol-fixed (100%, 5 min) HepG2 cells using a BMI-1 polyclonal antibody (Product # PA1-32487) at a dilution of 1:200 overnight at 4°C. Cells were blocked and permeabilized in 1%BSA/10% normal donkey serum/0.3M glycine in 0.1% PBS-Tween for 1hr prior to incubation with primary antibody. Detection was performed with a 488-conjugated secondary antibody and nuclei were stained using DAPI (blue). Plasma membranes were stained with WGA (red).
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide CDSGSDKANSPAGG, corresponding to amino acids 252-264 of Human Bmi1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32487 detects Bmi1 from human samples.
PA1-32487 has been successfully used in ELISA and Western blot applications.
The PA1-32487 immunogen is: Synthetic peptide: CDSGSDKANSPAGG, corresponding to amino acids 252-264 of Human Bmi1
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.