Immunohistochemical analysis of BMP2 in formalin-fixed paraffin-embedded babboon liver tissue using an anti-BMP2 polyclonal antibody (Product # PA1-31215) at a dilution of 1:100 using DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40x magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide PSDEVLSEFELRLLSM conjugated to KLH, corresponding to amino acids 45-60 of BMP2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:2000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31215 detects BMP2 from mouse and human samples.
PA1-31215 has been successfully used in ELISA, Dot blot, and Western blot applications. A band is detected around 45 kDa.
The PA1-31215 immunogen is: Synthetic peptide: PSDEVLSEFELRLLSM conjugated to KLH, corresponding to amino acids 45-60 of BMP2.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.