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Invitrogen
CD50 (ICAM-3) Monoclonal Antibody (CBR-IC3/1), Biotin, eBioscience™
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FIGURE: 1 / 2
CD50 (ICAM-3) Antibody (BMS111BT) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD41 FITC and CD50 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD50 clone CBR-IC3/1 stains lymphocytes (pink), monocytes (orange), granulocytes (blue) and a small subset of CD45 negative cells (green).
Product Details
BMS111BT
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CBR-IC3/1
Conjugate
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
2-8°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: Intercellular Adhesion Molecule-3 (ICAM-3) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1). ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.
Applications Tested: Flow Cytometry.
Purity: >95%.
Target Information
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD50; CDw50; ICAM-3; ICAM-R; intercellular adhesion molecule; Intercellular adhesion molecule 3; unnamed protein product
Gene Aliases: CD50; CDW50; ICAM-R; ICAM3
UniProt ID: (Human) P32942
Entrez Gene ID: (Human) 3385
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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