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Invitrogen

IL-10 Monoclonal Antibody (BT-10), eBioscience™

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View all (99) IL-10 antibodies

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Product Details

BMS131-2

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BT-10

Immunogen

Rec. Human IL-10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637193

Product Specific Information

Description: IL-10 (BT-10) is a mouse monoclonal antibody raised against IL-10 of human origin. BMS 131/2 is recommended for detection of IL-10 of human origin by solid phase ELISA.

Interleukin-10 is a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones.

Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

Purity is greater than 95% IgG1 as determined by SDS-PAGE.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; IL10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)
cytokine activity interleukin-10 receptor binding protein binding growth factor activity protein dimerization activity
Process(es)
negative regulation of cytokine production positive regulation of cytokine production positive regulation of endothelial cell proliferation response to molecule of bacterial origin chronic inflammatory response to antigenic stimulus positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response negative regulation of chronic inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process immune response negative regulation of cell proliferation response to xenobiotic stimulus regulation of gene expression negative regulation of autophagy response to activity response to inactivity hemopoiesis B cell differentiation leukocyte chemotaxis negative regulation of myeloid dendritic cell activation negative regulation of B cell proliferation response to lipopolysaccharide negative regulation of interferon-alpha production negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-6 production negative regulation of interleukin-8 production negative regulation of tumor necrosis factor production response to insulin negative regulation of heterotypic cell-cell adhesion positive regulation of heterotypic cell-cell adhesion response to carbon monoxide cellular response to hepatocyte growth factor stimulus type 2 immune response B cell proliferation negative regulation of T cell proliferation defense response to bacterium defense response to protozoan positive regulation of macrophage activation negative regulation of apoptotic process negative regulation of neuron apoptotic process negative regulation of nitric oxide biosynthetic process regulation of isotype switching negative regulation of MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of cell cycle positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade negative regulation of inflammatory response regulation of synapse organization negative regulation of membrane protein ectodomain proteolysis response to glucocorticoid negative regulation of cytokine activity branching involved in labyrinthine layer morphogenesis cellular response to lipopolysaccharide cellular response to estradiol stimulus negative regulation of chemokine (C-C motif) ligand 5 production endothelial cell apoptotic process liver regeneration interleukin-10-mediated signaling pathway positive regulation of plasma cell differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of response to wounding negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway regulation of complement-dependent cytotoxicity positive regulation of sprouting angiogenesis negative regulation of vascular smooth muscle cell proliferation positive regulation of vascular smooth muscle cell proliferation negative regulation of endothelial cell apoptotic process
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