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          • Primary Antibodies ›
          • Annexin V Antibodies

          Invitrogen

          Annexin V Monoclonal Antibody (VAA-33), eBioscience™

          Advanced Verification
          View all (36) Annexin V antibodies

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          Cite Annexin V Monoclonal Antibody (VAA-33), eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          • Advanced Verification (1)
          Annexin V Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Annexin V Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Annexin V Antibody (BMS147) in WB

          Western blot was performed using Annexin V Monoclonal Antibody (VAA-33), eBioscience™ (Product # BMS147) and a 35 kDa band corresponding to Anxa5 was observed and relative expression was seen in RD, BeWo and U-87 MG which are positive for Annexin V when compared to other cell lines. Whole cell extracts (40 µg lysate) of RD (Lane 1), BeWo (Lane 2), U-87 MG (Lane 3), Ramos (Lane 4), AML-193 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nit... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Annexin V Antibody in Western Blot (WB)
          Annexin V Antibody

          Product Details

          BMS147

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Flow Cytometry (Flow)

          Assay-Dependent
          -

          ELISA (ELISA)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          VAA-33

          Immunogen

          Rec. Human Annexin V

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • FITC
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2-7.4

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10597001

          Product Specific Information

          Description: BMS147 specifically recognizes Annexin V (VAC- alpha ). BMS147 is suitable for ELISA application, Western blot analysis and is suitable as primary antibody for staining in FACS analysis.

          BMS147FI can be used for direct staining of cells.

          BMS147BT can be used in a two-step procedure using the biotin-(strept-)avidin system. For intracellular stainings the use of BMS147FI is recommended.

          Applications Tested: ELISA, Flow Cytometry (Intracellular Staining), Western Blotting.

          Purity: >95%.

          Target Information

          The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Bioinformatics

          Protein Aliases: Anchorin CII; Annexin A5; Annexin V; Annexin-5; anticoagulant precursor (5' end put.); putative; ANXA5; Calphobindin I; CBP-I; CPB-I; Endonexin II; epididymis secretory protein Li 7; Lipocortin V; lipocortin-V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; unnamed protein product; VAC-alpha; Vascular anticoagulant-alpha

          View more View less

          Gene Aliases: ANX5; ANXA5; CPB-I; ENX2; HEL-S-7; PP4; RPRGL3; VAC-alph

          View more View less

          UniProt ID: (Human) P08758

          View more View less

          Entrez Gene ID: (Human) 308

          View more View less

          Function(s)
          phosphatidylserine binding phospholipase inhibitor activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding
          Process(es)
          signal transduction blood coagulation hemostasis negative regulation of apoptotic process negative regulation of coagulation
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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