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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CDC42EP3 Antibodies

          Bethyl Laboratories

          BORG2 Polyclonal Antibody

          View all (7) CDC42EP3 antibodies

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          Cite BORG2 Polyclonal Antibody

          • Antibody Testing Data (1)
          BORG2 Antibody in Immunoprecipitation (IP)
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          BORG2 Antibody in Immunoprecipitation (IP)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          BORG2 Antibody (A301-723A) in IP

          Detection of human BORG2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG2 antibody (Product # A301-723A) used for IP at 3 µg/mg lysate. BORG2 was also immunoprecipitated by rabbit anti-BORG2 antibody A301-722A, which recognizes a downstream epitope. For blotting immunoprecipitated BORG2, A301-723A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          BORG2 Antibody in Immunoprecipitation (IP)
          BORG2 Polyclonal Antibody

          Product Details

          A301-723A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          2-5 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 204 and 254 of human binder of Rho GTPases 2.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          phosphate/tris citrate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Based on 100% sequence identity, this antibody is predicted to react with Mouse

          Target Information

          CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Binder of Rho GTPases 2; CDC42 effector protein (Rho GTPase binding) 3; Cdc42 effector protein 3; CRIB-containing BORG2 protein; MSE55-related Cdc42-binding protein; MSE55-related protein

          View more View less

          Gene Aliases: BORG2; CDC42EP3; CEP3; UB1

          View more View less

          UniProt ID: (Human) Q9UKI2

          View more View less

          Entrez Gene ID: (Human) 10602

          View more View less

          Function(s)
          GTPase activator activity cytoskeletal regulatory protein binding GTP-Rho binding
          Process(es)
          signal transduction Rho protein signal transduction regulation of cell shape positive regulation of actin filament polymerization positive regulation of pseudopodium assembly positive regulation of GTPase activity
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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