|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues K(31) Q E R G E G P R A G E K G S H E E E P(50) of human BRAF35.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4192 detects BRAF35 in human samples.
PA1-4192 has been successfully used in Western blot procedures.
The PA1-4192 immunogen is a synthetic peptide corresponding to residues K(31) Q E R G E G P R A G E K G S H E E E P(50) of the human structural DNA-binding protein BRAF35. This sequence is 80% conserved in mouse.
Mutations of the human BRCA2 gene confer susceptibility to breast cancer. A structural DNA binding component has been identified and is designated as BRCA2-Associated Factor 35 (BRAF35). There is a close association of the BRAF35/BRCA2 complex with condensed chromatin coincident with histone H3 phosphorylation. Experimental evidence suggests a role for the BRCA2/BRAF35 complex in the modulation of cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.