Invitrogen

BRAF35 Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-148289

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human HMG20B fragment

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3247458

Target Information

Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRCA2-associated factor 35; FLJ26127; high-mobility group 20B; HMG box domain containing 2; HMG box-containing protein 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; SMARCE1-related protein; Sox-like transcriptional factor; Structural DNA-binding protein BRAF35; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

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Gene Aliases: B-raf; B-RAF1; BRAF; BRAF1; BRAF25; BRAF35; HMG20B; HMGX2; HMGXB2; NS7; PP7706; pp8857; RAFB1; SMARCE1R; SOXL

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UniProt ID: (Human) P15056, (Human) Q9P0W2

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Entrez Gene ID: (Human) 673, (Human) 10362

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding protein heterodimerization activity DNA binding histone deacetylase activity non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPKK activity myeloid progenitor cell differentiation protein phosphorylation visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration glucose transport thyroid gland development positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance cellular response to drug regulation of cell proliferation negative regulation of apoptotic process CD4-positive, alpha-beta T cell differentiation positive T cell selection response to peptide hormone negative regulation of neuron apoptotic process thymus development positive regulation of axon regeneration positive regulation of axonogenesis protein heterooligomerization positive regulation of stress fiber assembly response to cAMP long-term synaptic potentiation head morphogenesis face development positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion establishment of protein localization to membrane positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process transcription, DNA-templated regulation of transcription, DNA-templated cell cycle blood coagulation histone deacetylation negative regulation of protein sumoylation skeletal muscle cell differentiation positive regulation of neuron differentiation
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