Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | - |
Flow Cytometry (Flow) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
1:100 | View 1 publication 1 publication |
ChIP assay (ChIP) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to amino acids near the amino terminus of human BRCA1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
Contains |
no preservative |
Storage conditions |
-20°C |
RRID |
AB_10982612 |
It is not recommended to aliquot this antibody.
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: brca 1; brca-1; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; Breast cancer type 1 susceptibility protein; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; protein phosphatase 1, regulatory subunit 53; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein
Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
UniProt ID: (Human) P38398
Entrez Gene ID: (Human) 672
Molecular Function:
ubiquitin-protein ligase
protein modifying enzyme
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