|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: S(1046) S K K Q K R S H K(1055).|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4419 detects BRCAA1 in human samples.
PA1-4419 has been successfully used in Western blotting procedures. By Western blot, this antibody detects a ~136 kDa protein representing BRCAA1 in MCF-7 cell lysates.
PA1-4419 immunizing peptide corresponds to amino acid residues 1046-1055 of human BRCAA1.
The novel BRCAA1 protein and quote;s epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope exhibited positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.