Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.2-1 µg/mL | - |
Immunocytochemistry (ICC/IF) |
4 µg/mL | - |
ChIP assay (ChIP) |
0.5 µg | View 2 publications 2 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide directed towards the C-terminal of human BRD4 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS with 2% sucrose |
Contains |
0.09% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2610136 |
Peptide sequence: EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSS
Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRD4-NUT FUSION; bromodomain-containing 4; Bromodomain-containing protein 4; chromosome-associated protein; Protein HUNK1
Gene Aliases: BRD4; CAP; HUNK1; HUNKI; MCAP
UniProt ID: (Human) O60885
Entrez Gene ID: (Human) 23476
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