Invitrogen

BRD4 Polyclonal Antibody

Product Image Gallery

BRD4 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of HeLa cells using an anti-BRD4 polyclonal antibody (Product # PA5-41550). Primary Antibody Dilution: 4 µg/mL; Secondary Antibody: Anti-rabbit Alexa 546; Secondary Antibody Dilution: 2 µg/mL.

BRD4 Antibody in Western Blot (WB) — Western blot analysis of BRD4. 50 µg total protein lysate from human embryonic kidney HEK293T cells (lane 2) and mouse myoblast C2C12 cells (lane 3) in 1X NuPAGE® LDS Sample Buffer (Product # NP0007) + 1mM DTT loaded onto a NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (Product # NP0321PK2). Molecular weight marker loaded for size reference (lane 1). Gel run in 1X NuPAGE® MOPS SDS Running Buffer (Product # NP0001) at room temperature and then transferred to a methanol-activated Novex™ PVDF membrane (Product # LC2002) using a Invitrogen Mini Blot Module (Product # B1000) in 1X NuPAGE® Transfer Buffer (Product # NP0006) at 20V for 2 hours at room temperature. Membrane was rinsed in 1X TBS-T and incubated in blocking solution (5% milk in 1X TBS-T) for 1 hour at room temperature on a rocking platform. HDAC1 detection was performed by incubating blot with rabbit polyclonal anti-BRD4 antibody (Product # PA5-41550; 0.9 mg/mL) diluted at 1:1000 in blocking solution for 1 hour at room temperature on a rocking platform. Primary antibody was washed 10 times with 1X TBS-T over the course of 1 hour at room temperature on a rocking platform. Secondary antibody binding was performed by incubation with goat anti-rabbit IgG-HRP secondary diluted at 1:5000 in blocking solution for 1 hour at room temperature on a rocking platform. Following washes (as above), chemiluminescent imaging was performed. Data courtesy of Dr. Raj Jain's laboratory.

BRD4 Antibody in ChIP assay (CHIP) — ChIP assay analysis of HCT116 cells using an anti-BRD4 polyclonal antibody (Product # PA5-41550).

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

0.5 µg

Immunocytochemistry (ICC)

4 µg/mL

Immunofluorescence (IF)

4 µg/mL

Western Blot (WB)

0.2-1 µg/mL

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Rabbit, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the C-terminal of human BRD4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2610136

Product Specific Information

Peptide sequence: EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSS

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%

Target Information

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bromodomain containing 4; bromodomain-containing 4; bromodomain-containing 5; Bromodomain-containing protein 4; chromosome-associated protein; fsh-like protein; HUNK1; HUNKI; MCAP; Mitotic chromosome-associated protein; Protein HUNK1

Gene Aliases: BRD4; Brd5; CAP; HUNK1; HUNKI; MCAP; WI-11513

UniProt ID: (Human) O60885, (Mouse) Q9ESU6

Entrez Gene ID: (Dog) 484869, (Human) 23476, (Rabbit) 100352214, (Rat) 362844, (Guinea pig) 100717142, (Mouse) 57261

Molecular Function: DNA binding protein acetyltransferase chromatin/chromatin-binding protein nucleic acid binding transferase