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Invitrogen
BRIP1 Polyclonal Antibody
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FIGURE: 1 / 4
BRIP1 Antibody (PA5-27096) in ICC/IF
BRIP1 Polyclonal Antibody detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: BRIP1 protein stained by BRIP1 Polyclonal Antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.
Product Details
PA5-27096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human FANCJ. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BRIP1 (BRCA1 Interacting Protein C-terminal Helicase 1) is a gene situated on chromosome 17q22, covering 19 exons within its 184-kb coding region to encode a protein containing 1249 amino acids. BRIP1 is integral to the DNA repair processes, specifically through its interaction with the BRCA1 gene, and is involved in the Fanconi anemia complementation group J (FANC J). Mutations in BRIP1 have been identified, playing a role in cancer predisposition with a notable eightfold increased risk for ovarian cancer and associations with cervical cancer risk through its single nucleotide polymorphisms (SNPs). Amplifications and other mutations of BRIP1 are prevalent in a variety of cancers, and its expression involves significant correlations with copy number variations (CNV) and DNA methylation across numerous tumor types. BRIP1's expression is upregulated in certain contexts, which can contribute to cancer progression, as evidenced in the Omani population with breast cancer. These interactions validate its association with DNA damage repair, cell cycle, and metabolism across multiple cancer types.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase BRIP1; BACH 1; BACH1 BRIP1; BRCA1 interacting helicase 1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2-associated helicase 1; BRCT-binding helicase-like protein BACH1; BRIP 1; DNA 5'-3' helicase FANCJ; FANCJ helicase; FANCJ protein; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; unnamed protein product
Gene Aliases: BACH1; BRIP1; FANCJ; OF
UniProt ID: (Human) Q9BX63
Entrez Gene ID: (Human) 83990
nucleotide binding
nucleic acid binding
DNA binding
DNA helicase activity
RNA helicase activity
helicase activity
protein binding
ATP binding
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
isomerase activity
ATPase activity
5'-3' DNA helicase activity
metal ion binding
iron-sulfur cluster binding
4 iron, 4 sulfur cluster binding
catalytic activity, acting on a nucleic acid
DNA damage checkpoint
nucleobase-containing compound metabolic process
DNA repair
nucleotide-excision repair
double-strand break repair
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
synapsis
spermatogenesis
spermatogonial cell division
spermatid development
male gonad development
meiotic DNA double-strand break processing involved in reciprocal meiotic recombination
reciprocal DNA recombination
interstrand cross-link repair
chiasma assembly
seminiferous tubule development
protein-DNA covalent cross-linking repair
double-strand break repair involved in meiotic recombination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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