Immunofluorescent analysis of BRMS1L showing staining in the nucleus of H1299 cells. H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a BRMS1L polyclonal antibody (Product # PA5-32016) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 10 and 222 of Human BRMS1L|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32016 targets BRMS1L in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-32016 immunogen is recombinant protein fragment corresponding to a region within amino acids 10 and 222 of Human BRMS1L.
The protein encoded by this gene shows sequence similarity to the human breast carcinoma metastasis suppressor (BRMS1) protein and the mammalian Sds3 (suppressor of defective silencing 3) proteins. This protein is a component of the mSin3a family of histone deacetylase complexes (HDAC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.