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Phospho-Bad (Ser134) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-Bad (Ser134) Antibody (BS-5217R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bad (Ser134) Polyclonal Antibody, Unconjugated (bs-5217R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-5217R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser134.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAD; BBC6; Bcl-2-binding component 6; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; Bcl2-associated agonist of cell death; BCL2-binding component 6; BCL2-binding protein; Bcl2-L-8; OTTMUSP00000017561; OTTMUSP00000022400; unnamed protein product
Gene Aliases: BAD; BBC2; BBC6; BCL2L8
UniProt ID: (Human) Q92934
Entrez Gene ID: (Human) 572
release of cytochrome c from mitochondria
glucose catabolic process
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of autophagy
positive regulation of mitochondrial membrane potential
cytokine-mediated signaling pathway
positive regulation of insulin secretion
positive regulation of insulin secretion involved in cellular response to glucose stimulus
glucose homeostasis
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
type B pancreatic cell proliferation
positive regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of proteolysis
ADP metabolic process
ATP metabolic process
regulation of mitochondrial membrane permeability
pore complex assembly
epithelial cell proliferation
positive regulation of epithelial cell proliferation
cellular response to mechanical stimulus
cellular response to nicotine
cellular response to lipid
cellular response to hypoxia
positive regulation of release of cytochrome c from mitochondria
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
positive regulation of type B pancreatic cell development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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