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LC3A Monoclonal Antibody (7G10)
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FIGURE: 1 / 3
LC3A Antibody (BSM-33309M) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4°C, DAB staining.
Product Details
BSM-33309M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
7G10
Immunogen
KLH conjugated synthetic peptide derived from human LC3A
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.03% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3-like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-asso; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A; RP11-346K17.1
Gene Aliases: 1010001H21Rik; 4922501H04Rik; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1LC3A
UniProt ID: (Human) Q9H492, (Rat) Q6XVN8, (Mouse) Q91VR7
Entrez Gene ID: (Human) 84557, (Rat) 362245, (Mouse) 66734
autophagosome assembly
mitophagy
mitophagy
autophagy
cellular response to nitrogen starvation
JNK cascade
cellular response to starvation
response to iron(II) ion
response to lead ion
macroautophagy
response to nutrient levels
cellular response to amino acid starvation
p38MAPK cascade
SMAD protein signal transduction
cellular response to hydrogen peroxide
cellular response to copper ion
cellular response to oxygen-glucose deprivation
autophagosome maturation
nucleophagy
membrane fusion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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