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Bach2 Antibody (600-401-H35) in WB
Western Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 µg per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted (native)/Observed (over-expressed) size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none.
Product Details
600-401-H35
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Bach2 antibody is directed against mouse Bach2 (n-terminus) protein. A BLAST analysis was used to suggest 100% homology with Bach2 protein from mouse. Reactivity against homologues from other sources is not known.
Target Information
BTB and CNC homolog 2 (BACH2) is a transcriptional regulator that acts as repressor or activator. BACH2 binds to Maf recognition elements (MARE). It induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BTB and CNC homolog 2; Transcription regulator protein BACH2; Transcription regulator protein BACH2 (BTB and CNC homolog 2)
Gene Aliases: Bach2; E030004N02Rik
UniProt ID: (Mouse) P97303
Entrez Gene ID: (Mouse) 12014
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