Proteintech

Bcl-XL Polyclonal Antibody

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Bcl-XL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( ) fixed NIH/3T3 cells using 10783-1-AP (Bcl-XL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Bcl-XL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Bcl-XL Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

Bcl-XL Antibody in Flow Cytometry (Flow) — 1X10^6 Jurkat cells were stained with 0.20ug Bcl-XL antibody (10783-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor488-conjugated AfiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Bcl-XL Antibody in Immunoprecipitation (IP) — IP result of anti-Bcl-XL (IP:10783-1-AP, 3ug; Detection:10783-1-AP 1:500) with K-562 cells lysate 8000 ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 62 publications 62 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Bcl-XL Fusion Protein Ag1232

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can recognize Bcl-XL, Bcl-X (s) and Bcl-X(Beta).

Target Information

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BCLXL; BCLXS; DKFZp781P2092; OTTHUMP00000030550; OTTHUMP00000030552; OTTHUMP00000030555; OTTHUMP00000030556; OTTHUMP00000030557; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3

Gene Aliases: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; Blc2l; PPP1R52

UniProt ID: (Human) Q07817, (Mouse) Q64373, (Rat) P53563

Entrez Gene ID: (Human) 598, (Mouse) 12048, (Rat) 24888

Function(s)