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Agrisera
BiP/Lumenal-binding protein Polyclonal Antibody
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FIGURE: 1 / 2
BiP/Lumenal-binding protein Antibody (AS09-614) in IHC (PFA)
Bi P localization in 5 days old Arabidopsis thaliana roots. Bi P signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-Bi P primary antibody was diluted in 1:1,000 and Dy Light® 488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1:1,000. Co-stainin... View More
Product Details
AS09-614
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Chicken
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1, BiP2, BiP3.
Conjugate
Form
Liquid
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunogen affinity purified IgY
Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: Arabidopsis thaliana, Hordeum vulgare, Physcomitrium patens, Spinacia oleracea, Zea mays
Target Information
BiP is an HSPA5-encoded member of the Hsp70 chaperone family that resides primarily in the endoplasmic reticulum (ER) lumen via an N-terminal signal peptide that mediates ER import and a C-terminal ER-retention motif (KDEL); under stress it can also relocalize to additional compartments, including the cell surface. Structurally, BiP is a ~78 kDa ATP-dependent chaperone organized into a conserved N-terminal nucleotide-binding (ATPase) domain and a C-terminal substrate-binding domain with a lid region, enabling cycles of ATP binding and hydrolysis to regulate client engagement and release. Functionally, BiP is a central ER proteostasis factor that binds nascent and misfolded polypeptides to promote folding and assembly, helps maintain ER integrity, and supports disposal of aberrant proteins through ER-associated degradation; it also acts as a key regulator of the unfolded protein response by binding the luminal domains of ER stress sensors (including IRE1, PERK, and ATF6) under basal conditions and being titrated away by accumulating unfolded proteins to permit signaling that restores ER homeostasis or, if stress is unresolved, contributes to downstream cell fate decisions such as autophagy and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BIP; BiP-L; BIP1; BIP2; BIP3; luminal binding protein; MJC20.12; MJC20_12; T26D3.10; T26D3_10
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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