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Invitrogen
Bid Polyclonal Antibody, DyLight™ 488
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FIGURE: 1 / 1
Bid Antibody (PA5-78872) in Flow
Flow Cytometry of Bid in K562 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Bid Polyclonal Antibody, DyLight™ 488 (Product # PA5-78872) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
Product Details
PA5-78872
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Bid recombinant protein (Position: M1-D195).
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
200 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control - Flow: K562 cell.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BID; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID; unnamed protein product
Gene Aliases: BID; FP497
UniProt ID: (Human) P55957
Entrez Gene ID: (Human) 637
release of cytochrome c from mitochondria
protein targeting to mitochondrion
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
apoptotic mitochondrial changes
positive regulation of mitochondrial membrane potential
positive regulation of protein complex assembly
cellular response to stress
intracellular signal transduction
regulation of T cell proliferation
signal transduction in response to DNA damage
mitochondrial ATP synthesis coupled electron transport
regulation of apoptotic process
positive regulation of apoptotic process
regulation of epithelial cell proliferation
neuron apoptotic process
protein-containing complex assembly
establishment of protein localization to membrane
positive regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
mitochondrial outer membrane permeabilization
fibril organization
regulation of mitochondrial membrane permeability involved in apoptotic process
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
regulation of intracellular signal transduction
regulation of G1/S transition of mitotic cell cycle
positive regulation of fibroblast apoptotic process
regulation of apoptotic signaling pathway
positive regulation of apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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