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FIGURE: 1 / 4
Bif1 Antibody (PA5-28068) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Bif1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 10 min. Green: Bif1 Polyclonal Antibody (Product # PA5-28068) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-28068
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 302 and 365 of Human Bif1
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545544
Product Specific Information
Recommended positive controls: H1299, NIH-3T3, JC, BCL-1.^M
^M
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Bif1 gene encodes a protein similar to a protein in rodents which is induced by bone morphogenic protein 2 in vitro and may be involved in BMP2-induced transcription.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bax-interacting factor 1; Bif-1; BIF1; endophilin b1; Endophilin-B1; protein phosphatase 1, regulatory subunit 70; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2 like endophilin B1; SH3-domain GRB2-like endophilin B1; testicular tissue protein Li 172
Gene Aliases: AA409932; AI314629; AU015566; Bif-1; CGI-61; dJ612B15.2; KIAA0491; mKIAA0491; PPP1R70; SH3GLB1
UniProt ID: (Human) Q9Y371, (Mouse) Q9JK48
Entrez Gene ID: (Human) 51100, (Mouse) 54673
autophagy
apoptotic process
positive regulation of autophagy
regulation of macroautophagy
regulation of protein stability
positive regulation of protein oligomerization
regulation of cytokinesis
receptor catabolic process
cellular response to amino acid starvation
cellular response to glucose starvation
autophagic cell death
protein oligomerization
membrane fission
cell-cell adhesion
positive regulation of membrane tubulation
protein localization to vacuolar membrane
positive regulation of protein targeting to mitochondrion
positive regulation of autophagosome assembly
phosphatidic acid biosynthetic process
mitochondrion organization
phospholipid biosynthetic process
positive regulation of phosphatidylinositol 3-kinase activity
'de novo' posttranslational protein folding
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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