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          • Primary Antibodies ›
          • Biglycan Antibodies

          Invitrogen

          Biglycan Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          5 References
          View all (14) Biglycan antibodies

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          Cite Biglycan Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (4)
          • Advanced Verification (1)
          Biglycan Antibody in Western Blot (WB)
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          Biglycan Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          Biglycan Antibody (PA5-76821) in WB

          Western blot was performed using Anti-Biglycan Polyclonal Antibody (Product # PA5-76821) and a 42 kDa bands corresponding to Biglycan was observed in Mouse Heart but not in Mouse Skeletal Muscle. An uncharacterized bands of ~23 kDa was observed in negative cell line. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1) and Mouse Small Intestine (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Biglycan Antibody in Western Blot (WB)
          Biglycan Antibody in Western Blot (WB)
          Biglycan Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)
          Biglycan Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)
          Biglycan Antibody in Western Blot (WB)
          Biglycan Antibody in Western Blot (WB)
          Biglycan Antibody
          Biglycan Polyclonal Antibody

          Product Details

          PA5-76821

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant full length Human Biglycan.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2720548

          Product Specific Information

          The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

          Target Information

          BGN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transferring growth factor-beta. It may promote neuronal survival.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Biglycan; bone/cartilage proteclycan 1; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; PG S1; PG-S1; proteoglycan I precursor; small leucine-rich protein 1A; Small proteoglycan I (biglycan), bone (BSPG1) (bone/cartilage proteclycan 1 precursor); unnamed protein product

          View more View less

          Gene Aliases: BG; BGN; BSPG1; DSPG1; MRLS; PG-S1; PGI; SEMDX; SLRR1A

          View more View less

          UniProt ID: (Human) P21810, (Mouse) P28653, (Rat) P47853

          View more View less

          Entrez Gene ID: (Human) 633, (Mouse) 12111, (Rat) 25181

          View more View less

          Function(s)
          extracellular matrix structural constituent protein binding glycosaminoglycan binding cytokine binding extracellular matrix structural constituent conferring compression resistance extracellular matrix binding
          Process(es)
          peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan blood vessel remodeling bone development articular cartilage development
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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