A suggested positive control is K562 cell lysate.
PA5-20089 can be used with blocking peptide PEP-0207.
The PA5-20089 immunogen is located within amino acids 80-130 of BIM.
Predicted molecular ~ 22kD. In Western blot applications, this antibody has been observed to detect a band at: 22kD.
Predicted species reactivity based on immunogen sequence: Rat: (100%).
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
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Protein Aliases: BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BOD, BimEL, BimL; bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; Bcl-2-like protein 11; bcl-2-related ovarian death agonist; Bcl2 interacting mediator of cell death; Bcl2-interacting mediator of cell death; Bcl2-L-11; BCL2-like 11 (apoptosis facilitator)
Gene Aliases: 1500006F24Rik; BAM; bcl2-L-11; BCL2L11; BIM; BOD