A suggested positive control is K562 cell lysate.
PA5-20089 can be used with blocking peptide PEP-0207.
The PA5-20089 immunogen is located within amino acids 80-130 of BIM.
Predicted molecular ~ 22kD. In Western blot applications, this antibody has been observed to detect a band at: 22kD.
Predicted species reactivity based on immunogen sequence: Rat: (100%).
BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.
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Protein Aliases: bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; Bcl-2 like 11; Bcl-2-like protein 11; bcl-2-related ovarian death agonist; Bcl2 interacting mediator of cell death; Bcl2-interacting mediator of cell death; Bcl2-L-11; BCL2-like 11 (apoptosis facilitator); Bim EL; Bim-A; BIM-alpha6; BIM-beta6; BIM-beta7; OTTHUMP00000197013; OTTHUMP00000204247
Gene Aliases: 1500006F24Rik; BAM; bcl2-L-11; BCL2L11; BIM; BOD