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Bim Antibody (PA5-20089) in ICC/IF
Immunocytochemistry of K562 cells using Bim Polyclonal Antibody (Product # PA5-20089) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Product Details
PA5-20089
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to amino acids near the center of human Bim
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152570
Product Specific Information
A suggested positive control is K562 cell lysate.
PA5-20089 can be used with blocking peptide PEP-0207.
The PA5-20089 immunogen is located within amino acids 80-130 of BIM.
Predicted molecular ~ 22kD. In Western blot applications, this antibody has been observed to detect a band at: 22kD.
Predicted species reactivity based on immunogen sequence: Rat: (100%).
Target Information
BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; Bcl-2 like 11; Bcl-2-like protein 11; bcl-2-related ovarian death agonist; Bcl2 interacting mediator of cell death; Bcl2-interacting mediator of cell death; Bcl2-L-11; BCL2-like 11 (apoptosis facilitator); Bim EL; Bim-A; BIM-alpha6; BIM-beta6; BIM-beta7; OTTHUMP00000197013; OTTHUMP00000204247
Gene Aliases: 1500006F24Rik; BAM; bcl2-L-11; BCL2L11; BIM; BOD
UniProt ID: (Human) O43521, (Mouse) O54918
Entrez Gene ID: (Human) 10018, (Mouse) 12125
in utero embryonic development
leukocyte homeostasis
B cell homeostasis
B cell apoptotic process
kidney development
lymphocyte homeostasis
myeloid cell homeostasis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell-matrix adhesion
spermatogenesis
male gonad development
intrinsic apoptotic signaling pathway in response to DNA damage
post-embryonic development
positive regulation of cell death
mammary gland development
positive regulation of protein homooligomerization
response to endoplasmic reticulum stress
tube formation
odontogenesis of dentin-containing tooth
regulation of apoptotic process
T cell homeostasis
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of neuron apoptotic process
ear development
positive regulation of cell cycle
regulation of organ growth
developmental pigmentation
regulation of developmental pigmentation
spleen development
thymus development
post-embryonic organ morphogenesis
positive regulation of apoptotic process by virus
cellular process regulating host cell cycle in response to virus
thymocyte apoptotic process
positive regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of mitochondrial membrane permeability involved in apoptotic process
apoptotic process involved in embryonic digit morphogenesis
positive regulation of fibroblast apoptotic process
positive regulation of intrinsic apoptotic signaling pathway
protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
meiosis I
brain development
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of IRE1-mediated unfolded protein response
Performance Guarantee
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