|Tested species reactivity||Chemical|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.8, with sucrose|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:25,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
The activity measured by a solid phase binding assay is 94%.
Reconstitute using 1 ml of distilled water; product may be stored for several weeks as an undiluted liquid. After dilution, do not use for more than one day. After one year, the reconstituted liquid product should be stored frozen in small undiluted aliquots, avoiding freeze/thaw cycles.
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Alkylation of the tumor suppressor PTEN activates Akt and ß-catenin signaling: a mechanism linking inflammation and oxidative stress with cancer.
31852 was used in western blot to investigate the effects of PTEN alkylation
|Covey TM,Edes K,Coombs GS,Virshup DM,Fitzpatrick FA||PloS one (5:null)||2010|