Invitrogen

Blood Group Lewis A Monoclonal Antibody (7LE)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (4) Blood Group Lewis A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Blood Group Lewis A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Blood Group Lewis A was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Blood Group Lewis A Monoclonal Antibody (7LE) (Product # MA1-19344) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane and cytoplasmic localization. Panel e shows MCF-7 cells with no expression of Blood Group Lewis A. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Blood Group Lewis A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry staining of human colon adenocarcinoma (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE) Monoclonal antibody (Product # MA1-19344).

Blood Group Lewis A Antibody in Western Blot (WB) — Western blot was performed using Anti-Blood Group Lewis A Monoclonal Antibody (Product # MA1-19344) and bands spanning between 60-200 kDa corresponding to glycosylated forms of Blood Group Lewis A were observed in A-431 and were absent in SK-BR-3, MCF-7 and PC-3 cell lysates. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), SK-BR-3 (Lane 2), MCF-7 (Lane 3) and PC-3 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Blood Group Lewis A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of Blood Group Lewis A was observed in A-431 as compared to SK-BR-3, MCF-7 and PC-3 using Anti-Blood Group Lewis A Monoclonal Antibody (Product # MA1-19344) in Western Blot. {RE}

Product Details

MA1-19344

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
Immunohistochemistry (Paraffin) (IHC (P))	5 µg/mL	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
ELISA (ELISA)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	7LE
Immunogen	Mucin from ovarian cyst fluid. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	15mM sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_1070784

Product Specific Information

For IHC (P), heat mediated antigen retrieval in sodium citrate is recommended.

Target Information

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems.

Glycobiology

Manimala JC,Roach TA,Li Z,Gildersleeve JC

MA1-19344 was used in ELISA to study the specificity of a range of antiglycan antibodies

Wed Aug 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3; 4-fucosyltransferase; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha-(1,3/1,4)-fucosyltransferase; Alpha-3-fucosyltransferase FUT3; Blood group Lewis alpha-4-fucosyltransferase; Fucosyltransferase 3; fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group); Fucosyltransferase III; FucT-III; Lewis FT; Lewis Y

Gene Aliases: CD174; FT3B; FucT-III; FUT3; LE; Les

UniProt ID: (Human) B5U7U9

Entrez Gene ID: (Human) 2525

Molecular Function: glycosyltransferase

Function(s)

protein N-linked glycosylation oligosaccharide biosynthetic process cell-cell recognition fucosylation macromolecule glycosylation

Process(es)

fucosyltransferase activity 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity alpha-(1->3)-fucosyltransferase activity

Blood Group Lewis A Monoclonal Antibody (7LE)

Product Details

MA1-19344

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!