|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Host / Isotype||Yeast|
|Storage conditions||4° C|
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant bovine TNFa was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D. The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 28.4 – 42.7 ng/ml.
Expression: The mature, soluble form of bovine TNF-alpha was expressed in Pichia pastoris, KM71H. GenBank® Accession # Q06599, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: LRSSSQASSN.
Reconstitute with 0.5 ml of distilled water.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bovine Tumor Necrosis Factor alpha TNFA; Cachectin; TNF-a; TNF-alpha; Tumor necrosis factor; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2
Gene Aliases: BOS_21587; TNF; TNF-a; TNF-alpha; TNFA; TNFSF2
UniProt ID: (Bovine) Q06599
Entrez Gene ID: (Bovine) 280943
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