Immunofluorescent analysis of BrdU showing staining in the nucleus of HeLa cells. HeLa cells mock (left) and BrdU treated with 50µM BrdU for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min and stained using a BrdU polyclonal antibody (Product # PA5-32256) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Chemical|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BrdU conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-32256 targets BrdU in immunofluorescence applications and shows reactivity with Human samples.
The PA5-32256 immunogen is brdU conjugated to KLH.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sonic hedgehog promotes stem-cell potential of Müller glia in the mammalian retina.
PA5-32256 was used in immunohistochemistry to study the role of sonic hedgehog signaling in the display of stem cell-like properties by Müller glia following retinal injury
|Wan J,Zheng H,Xiao HL,She ZJ,Zhou GM||Biochemical and biophysical research communications (363:347)||2007|