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C3/C3b/C3c Polyclonal Antibody

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C3/C3b/C3c Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

C3/C3b/C3c Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed human liver cancer tissue using 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

C3/C3b/C3c Antibody in Western Blot (WB) — Human plasma tissue were subjected to SDS PAGE followed by western blot with 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

C3/C3b/C3c Antibody in Immunoprecipitation (IP) — IP result of anti-C3/C3b/C3c (IP:21337-1-AP, 4ug; Detection:21337-1-AP 1:1000) with human plasma tissue lysate 4000 ug.

Product Details

21337-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	C3/C3b/C3c Fusion Protein Ag15537 (1314-1663 aa encoded by BC150179) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

Immunogen sequence: ESASLLRSE ETKENEGFTV TAEGKGQGTL SVVTMYHAKA KDQLTCNKFD LKVTIKPAPE TEKRPQDAKN TMILEICTRY RGDQDATMSI LDISMMTGFA PDTDDLKQLA NGVDRYISKY ELDKAFSDRN TLIIYLDKVS HSEDDCLAFK VHQYFNVELI QPGAVKVYAY YNLEESCTRF YHPEKEDGKL NKLCRDELCR CAEENCFIQK SDDKVTLEER LDKACEPGVD YVYKTRLVKV QLSNDFDEYI MAIEQTIKSG SDEVQVGQQR TFISPIKCRE ALKLEEKKHY LMWGLSSDFW GEKPNLSYII GKDTWVEHWP EEDECQDEEN QKQCQDLGAF TESMVVFGCP N (1314-1663 aa encoded by BC150179)

Target Information

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Surface-Exposed Protein SntA Contributes to Complement Evasion in Zoonotic Streptococcus suis.

Frontiers in immunology

Deng S,Xu T,Fang Q,Yu L,Zhu J,Chen L,Liu J,Zhou R

Wed Jul 24 00:00:00 EDT 2019

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Cardiology in the young

Zhang L,Song QF,Jin JJ,Huang P,Wang ZP,Xie XF,Gu XQ,Gao XJ,Jia HL

Wed Nov 01 00:00:00 EDT 2017

Serum estradiol levels in controlled ovarian stimulation directly affect the endometrium.

Journal of molecular endocrinology

Ullah K,Rahman TU,Pan HT,Guo MX,Dong XY,Liu J,Jin LY,Cheng Y,Ke ZH,Ren J,Lin XH,Qiu XX,Wang TT,Huang HF,Sheng JZ

Tue Aug 01 00:00:00 EDT 2017

Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling.

International journal of molecular sciences

Wu Z,Ding N,Yu M,Wang K,Luo S,Zou W,Zhou Y,Yan B,Jiang Q

Wed Dec 07 00:00:00 EST 2016

Bioinformatics

Protein Aliases: Acylation stimulating protein; acylation-stimulating protein cleavage product; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component C3a; complement component C3b; ENCF-1; ENCF-2; epididymis secretory sperm binding protein Li 62p; HSE-MSF; Neutrophil chemotactic factor-1; Neutrophil chemotactic factor-2; prepro-C3

Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp

UniProt ID: (Rat) P01026, (Human) P01024, (Mouse) P01027

Entrez Gene ID: (Rat) 24232, (Human) 718, (Mouse) 12266

Molecular Function: protease inhibitor

Function(s)

Process(es)

endopeptidase inhibitor activity protein binding lipid binding C5L2 anaphylatoxin chemotactic receptor binding cofactor binding serine-type endopeptidase activity receptor binding

C3/C3b/C3c Polyclonal Antibody

Product Details

21337-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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