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          • Primary Antibodies ›
          • CACNA2D2 Antibodies

          Invitrogen

          CACNA2D2 Polyclonal Antibody

          1 Reference
          View all (11) CACNA2D2 antibodies

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          Cite CACNA2D2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          CACNA2D2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          CACNA2D2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          CACNA2D2 Antibody (PA5-95536) in WB

          Western blot analysis of CACNA2D2 in Lane 1: human MCF-7 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with CACNA2D2 polyclonal antibody (Product # PA5-95536) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CACNA2D2 Antibody in Western Blot (WB)
          CACNA2D2 Antibody in Flow Cytometry (Flow)
          CACNA2D2 Polyclonal Antibody

          Product Details

          PA5-95536

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          0.5-1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          0.5-1 µg/mL
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human CACNA2D2 recombinant protein (Position: Q66-Q284).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807338

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human MCF-7 whole cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: alpha 2 delta calcium channel subunit; alpha2delta-2; calcium channel, voltage dependent, alpha2/delta subunit 2; calcium channel, voltage-dependent, alpha 2/delta subunit 2; Cav alpha2/delta2; ducky; gene 26; Protein ducky; Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2

          View more View less

          Gene Aliases: a2d2; CACNA2D; CACNA2D2; CASVDD; du; KIAA0558; mKIAA0558; td; torpid

          View more View less

          UniProt ID: (Human) Q9NY47, (Mouse) Q6PHS9, (Rat) Q8CFG6

          View more View less

          Entrez Gene ID: (Human) 9254, (Mouse) 56808, (Rat) 300992

          View more View less

          Function(s)
          voltage-gated calcium channel activity calcium channel activity metal ion binding voltage-gated ion channel activity calcium channel regulator activity voltage-gated ion channel
          Process(es)
          ion transport calcium ion transport neuromuscular junction development ion transmembrane transport organ growth regulation of multicellular organism growth positive regulation of organ growth muscle cell development rhythmic synaptic transmission calcium ion transmembrane transport regulation of neurotransmitter receptor localization to postsynaptic specialization membrane transport regulation of ion transmembrane transport muscle fiber development regulation of calcium ion transport
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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