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Rockland
CAD Polyclonal Antibody
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FIGURE: 1 / 3
CAD Antibody (600-401-AA4) in ICC/IF
Immunofluorescence Microscopy of CAD antibody. Tissue: Mouse kidney. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CAD antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CAD is nuclear and cytoplasmic. Staining: CAD as green fluorescent signal.
Product Details
600-401-AA4
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-CAD antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 17 amino acids near the internal region of murine CAD.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAD protein; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; carbamyl phosphatate synthetase 2; Multifunctional protein CAD
Gene Aliases: 2410008J01Rik; AU018859; Cad; Cpad
UniProt ID: (Mouse) B2RQC6
Entrez Gene ID: (Mouse) 69719, (Rat) 24240
nucleotide binding
UTP binding
catalytic activity
aspartate carbamoyltransferase activity
carbamoyl-phosphate synthase (ammonia) activity
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
dihydroorotase activity
protein kinase activity
ATP binding
zinc ion binding
amino acid binding
transferase activity
carboxyl- or carbamoyltransferase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
ligase activity
enzyme binding
identical protein binding
metal ion binding
hydrolase
ligase
urea cycle
'de novo' pyrimidine nucleobase biosynthetic process
pyrimidine nucleotide biosynthetic process
UTP biosynthetic process
cellular amino acid metabolic process
arginine biosynthetic process
glutamine metabolic process
nitrogen compound metabolic process
metabolic process
drug metabolic process
peptidyl-threonine phosphorylation
protein autophosphorylation
liver development
glutamine catabolic process
heart development
female pregnancy
lactation
response to amine
response to caffeine
organ regeneration
response to testosterone
cellular response to drug
response to cortisol
carbamoyl phosphate biosynthetic process
cellular response to epidermal growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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