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Invitrogen
CALCOCO2 Monoclonal Antibody (9E2F2)
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FIGURE: 1 / 3
CALCOCO2 Antibody (MA5-49291) in ICC/IF
Immunocytochemistry analysis of CALCOCO2 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CALCOCO2 Monoclonal antibody (Product # MA5-49291) using a dilution of 5 μg/mL. Cy3 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49291
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
9E2F2
Immunogen
E.coli-derived human NDP52/CALCOCO2 recombinant protein (Position: M1-L446).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human Hela whole cell, human Jurkat whole cell, human A549 whole cell, rat testis tissue, mouse testis tissue. ICC/IF: U20S cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen nuclear dot 52 kDa protein; calcium binding and coiled-coil domain 2; calcium-binding and coiled-coil domain-containing protein 2; NDP52; nuclear domain 10 protein 52; nuclear domain 10 protein 52 like 1; nuclear domain 10 protein NDP52; nuclear dot protein 52; unnamed protein product
Gene Aliases: 2410154J16Rik; NDP52; Ndp52l1; RGD1559974
UniProt ID: (Human) Q13137, (Mouse) A2A6M5
Entrez Gene ID: (Human) 10241, (Mouse) 76815, (Rat) 303479
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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