Invitrogen

CALCRL Polyclonal Antibody

Product Image Gallery

CALCRL Antibody in Western Blot (WB) — Western blot analysis of CALCRL was performed by loading (from left to right): Human lung cancer tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a CALCRL antibody (Product # PA5-50644) at a 1/550 dilution for 40 seconds, followed by a secondary antibody at 1/8000.

CALCRL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CALCRL in paraffin-embedded Human thyroid cancer tissue. Samples were probed using a CALCRL polyclonal antibody (Product # PA5-50644) at a dilution of 1/40.

CALCRL Antibody — Fig. 3 NP time-dependent induced changes in the expression of neurokinin and CGRP receptors in the MDA-MB-231 LUC+ cells homogenate using Western blot analysis. a Representative immunoblots for NK1R (~ 75-100 kDa), NK2R (~ 44 kDa), RAMP1 (~ 17 kDa), CALCRL (~ 45-55 kDa) and GAPDH (~ 37 kDa) from cells homogenates. b Changes in the expression of NK-1R, NK-2R, RAMP1 and CALCRL (first, second, third and fourth panel, respectively) in human metastatic breast cancer cells at 24-72 h after NP treatment. Three independent experiments were performed. Data represent NK-1R, NK-2R, RAMP1 and CALCRL/GAPDH ratio, normalized to the mean value for the corresponding control group at 24, 48 or 72 h (punctuated line). Data are presented as median (numbers and horizontal line) with boxes representing the 25 and 75 percentiles, * P < 0.05; ** P < 0.01; *** P < 0.001 compared to control or NP treatment group

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human

Published species

Artificial Control, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region derived from internal residues of human calcitonin receptor-like

Conjugate

Unconjugated

Form

Liquid

Concentration

2.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2636097

Product Specific Information

The antibody detects endogenous levels of total CALCRL / CRLR protein.

Target Information

CRLR is a receptor for calcitonin gene-related peptide type 1. The activity of CRLR is mediated by G proteins which activate adenylyl cyclase. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B-cell/lung/testis, bome marrow, embryo, lung and synovium libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Calcitonin gene-related peptide type 1 receptor; Calcitonin receptor-like receptor; CGRP receptor; CGRP type 1 receptor; Cgrp1 receptor

Gene Aliases: CALCRL; CGRPR; CRLR

UniProt ID: (Human) Q16602

Entrez Gene ID: (Human) 10203

Molecular Function: G-protein coupled receptor antibacterial response protein defense/immunity protein receptor