Invitrogen

CALCRL Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (9) CALCRL antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CALCRL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CALCRL in paraffin-embedded Human thyroid cancer tissue. Samples were probed using a CALCRL polyclonal antibody (Product # PA5-50644) at a dilution of 1/40.

CALCRL Antibody in Western Blot (WB) — Western blot analysis of CALCRL was performed by loading (from left to right): Human lung cancer tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a CALCRL antibody (Product # PA5-50644) at a 1/550 dilution for 40 seconds, followed by a secondary antibody at 1/8000.

CALCRL Antibody — Fig. 3 NP time-dependent induced changes in the expression of neurokinin and CGRP receptors in the MDA-MB-231 LUC+ cells homogenate using Western blot analysis. a Representative immunoblots for NK1R (~ 75-100 kDa), NK2R (~ 44 kDa), RAMP1 (~ 17 kDa), CALCRL (~ 45-55 kDa) and GAPDH (~ 37 kDa) from cells homogenates. b Changes in the expression of NK-1R, NK-2R, RAMP1 and CALCRL (first, second, third and fourth panel, respectively) in human metastatic breast cancer cells at 24-72 h after NP treatment. Three independent experiments were performed. Data represent NK-1R, NK-2R, RAMP1 and CALCRL/GAPDH ratio, normalized to the mean value for the corresponding control group at 24, 48 or 72 h (punctuated line). Data are presented as median (numbers and horizontal line) with boxes representing the 25 and 75 percentiles, * P < 0.05; ** P < 0.01; *** P < 0.001 compared to control or NP treatment group {TM}

Product Details

PA5-50644

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region derived from internal residues of human calcitonin receptor-like
Conjugate	Unconjugated
Form	Liquid
Concentration	2.7 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 40% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2636097

Product Specific Information

The antibody detects endogenous levels of total CALCRL / CRLR protein.

Target Information

CRLR is a receptor for calcitonin gene-related peptide type 1. The activity of CRLR is mediated by G proteins which activate adenylyl cyclase. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B-cell/lung/testis, bome marrow, embryo, lung and synovium libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuropeptide-induced modulation of carcinogenesis in a metastatic breast cancer cell line (MDA-MB-231^LUC+).

Cancer cell international

Gutierrez S,Boada MD

PA5-50644 was used in Western Blotting to establish that exposure of a breast cancer cell line to neuropeptides increases its ability to migrate and invade new tissues whilst also supporting the pro nociceptive and cancer promoter role of the peripheral nervous system, during the initial stages of the disease.

Wed Mar 30 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: Calcitonin gene-related peptide type 1 receptor; Calcitonin receptor-like receptor; CGRP receptor; CGRP type 1 receptor; Cgrp1 receptor

Gene Aliases: CALCRL; CGRPR; CRLR

UniProt ID: (Human) Q16602

Entrez Gene ID: (Human) 10203

Molecular Function: transmembrane signal receptor