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Invitrogen
CALM Polyclonal Antibody
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FIGURE: 1 / 1
CALM Antibody (PA5-87994) in WB
Western blot analysis of CALM in extracts of various cell lines. Samples were incubated with CALM Polyclonal antibody (Product # PA5-87994) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87994
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_0010086601)
Conjugate
Form
Liquid
Concentration
1.53 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: IKDSTAASRA TTLSNAVSSL ASTGLSLTKV DEREKQAALE EEQARLKALK EQRLKELAKK PHTSLTTAAS PVSTSAGGIM TAPAIDIFST PSSSNSTSKL PNDLLDLQQP TFHPSVHPMS TASQVASTWG GFTPSPVAQP HPSAGLNVDF ESVFGNKSTN VIVDSGGFDE L; Positive Samples: U-251MG, HepG2, MCF7, HeLa, Mouse lung; Cellular Location: Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus, clathrin-coated pit, clathrin-coated vesicle
Target Information
PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CALM; Clathrin assembly lymphoid myeloid leukemia; Clathrin assembly lymphoid myeloid leukemia protein; Phosphatidylinositol-binding clathrin assembly protein
Gene Aliases: CALM; CLTH; fit-1; Fit1; LAP; mKIAA4114; PAP180; PICALM
UniProt ID: (Human) Q13492, (Mouse) Q7M6Y3
Entrez Gene ID: (Human) 8301, (Mouse) 233489
protein complex assembly
receptor-mediated endocytosis
cell proliferation
negative regulation of gene expression
synaptic vesicle maturation
vesicle-mediated transport
endosomal transport
regulation of endocytosis
receptor internalization
regulation of protein localization
cargo loading into vesicle
positive regulation of transcription, DNA-templated
negative regulation of receptor-mediated endocytosis
clathrin coat assembly
iron ion homeostasis
clathrin-mediated endocytosis
iron ion import into cell
cell-cell adhesion
positive regulation of neuron death
positive regulation of beta-amyloid formation
regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process
endocytosis
multicellular organism development
axonogenesis
hemopoiesis
dendrite morphogenesis
regulation of protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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