Western blot analysis of CAP32 using a CAPZB polyclonal antibody (Product # PA5-12520). Dictyostelium discoideum FAM21 contains a Capping Protein Interaction site (CPI) at amino acids 1201-1217. A construct was created where this site was removed from FAM21, and both the full length and FAM21deltaCPI were tagged with GFP and expressed in D. discoideum cells. GFP beads were used to purify both constructs and western blotting was used to detect the presence of CAP32 (D. discoideum Capping Protein subunit). A GFP only construct was used as a control. It is shown here that CAP32 is pulled down with the full length protein but not when the interaction site is removed.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 54-81 amino acids from the N-terminal region of human CAPZB|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, mouse, porcine and rat based on sequence homology.
CAPZB is a member of the F-actin capping protein family. This protein is the beta subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.