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CARD10 Antibody (PA5-29261) in IHC (P)
CARD10 Polyclonal Antibody detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 Polyclonal Antibody (Product # PA5-29261) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-29261
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 341 and 630 of Human CARD10
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal, 0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546737
Product Specific Information
Recommended positive controls: 293T, H1299, Molt-4, Raji, mouse heart.^M
^M
Predicted reactivity: Mouse (94%), Rat (93%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA3 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-Kappa-B activation. When expressed in cells, this protein binds to BCL10 and activates NF-Kappa-B Recent experiments have shown that CARMA3 is required for EGF-induced NF-Kappa-B activation and contributes to tumor growth in vivo, suggesting that CARMA3 may serve as a new therapeutic target for the treatment of EGFR-driven tumors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl10 binding protein and activator of NFKB; Bcl10-interacting MAGUK protein 1; Bimp1; CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; Carma 3; caspase recruitment domain family, member 10; Caspase recruitment domain-containing protein 10
Gene Aliases: AI449026; BIMP1; CARD10; CARMA3
UniProt ID: (Human) Q8NC81, (Mouse) P58660
Entrez Gene ID: (Human) 29775, (Mouse) 105844
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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