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FIGURE: 1 / 1
CARD14 Antibody (PA5-120462) in WB
Western blot analysis of CARD14 in extracts of various cell lines. Samples were incubated with CARD14 Polyclonal antibody (Product # PA5-120462) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120462
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human CARD14 (NP_001244899.1).
Conjugate
Form
Liquid
Concentration
2.12 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914034
Product Specific Information
Positive test controls include: HT-29, HeLa. The target is usually found in the following locations: Cytoplasm, Cytoplasm.^M
^M
Immunogen sequence: SLSLHYSNAL QEKELAASRC RSLQEELYLL KQELQRANMV SSCELELQEQ SLRTASDQES GDEELNRLKE ENEKLRSLTF SLAEKDILEQ SLDEARGSRQ ELVERIHSLR ERAVAAERQR EQYWEEKEQT LLQFQKSKMA CQLYREKVNA LQAQVCELQK ERDQAYSARD SAQREISQSL VEKDSLRRQV FELTDQVCEL RTQLRQLQAE PPGVLKQEAR TREPCPREKQ RLVRMHAICP RDDSDCSLVS STESQLLSDL SATSSRELVD SFRSSSPAPP SQQSLYKRVA EDFGEEPWSF S
Target Information
Membrane-associated guanylate kinase (MAGUK) family members localize to the plasma membrane and function as molecular scaffolds for the assembly of multi-protein complexes. The MAGUK family includes several mammalian proteins related to the Drosophila tumor suppressor discs-large (dlg) gene product, such as postsynaptic proteins, GKAPs, the tight junction associated proteins (ZO-1-3) and the caspase-associated recruitment domain (CARD) proteins: CARD 6, CARD 8-12 and CARD 14. CARD 14 is a 1,004 amino acid protein consisting of an N-terminal CARD domain, a central coiled-coil domain and a C-terminal tripartite domain comprised of a PDZ domain, an Src homology 3 domain and a GUK domain with homology to guanylate kinase. CARD 14 is expressed in the placenta where it positively regulates apoptosis. CARD 14 also controls NF-kappaB activation by phosphorylating BCL10, a signaling protein that activates NF-kappaB through the I kappaB kinase complex. Epigallocatechin-3-gallate (EGCG) is a polyphenol that induces the expression of CARD 14.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl10-interacting MAGUK protein 2; Bimp2; CARD-containing MAGUK protein 2; Carma 2; caspase recruitment domain family, member 14; Caspase recruitment domain-containing protein 14
Gene Aliases: BIMP2; CARD14; CARMA2; PRP; PSORS2; PSS1
UniProt ID: (Human) Q9BXL6, (Mouse) Q99KF0
Entrez Gene ID: (Human) 79092, (Mouse) 170720, (Rat) 688292
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