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CARD8 Antibody (PA5-75635) in WB
Western blot was performed using CARD8 Polyclonal Antibody (Product # PA5-75635) and a 61 kDa corresponding to CARD8 full length form and 28 kDa corresponding to C-terminal fragment of CARD8 was observed across cell lines and tissues tested with testis showing higher expression when compared to other tissues. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), K-562 (Lane 2), MCF7 (Lane 3), Jurkat (Lane 4), HEK-293 (Lane 5) in Fig. a; Mouse Spleen (Lane 1), Mouse Lung (Lane 2), Mouse Placenta (Lane 3), Mouse Testis (Lane 4), Mouse Brain... View More
Product Details
PA5-75635
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human CARD8.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2719363
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-kappa-B activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptotic protein NDPP1; CARD 8; CARD inhibitor of NF-kappaB-activating ligands; CARD-inhibitor of NF-kappa-B-activating ligand; CARD8-CT; CARD8-NT; CARDINAL; caspase recruitment domain family, member 8; Caspase recruitment domain-containing protein 8; Caspase recruitment domain-containing protein 8, C-terminus; Caspase recruitment domain-containing protein 8, N-terminus; DKFZp779L0366; KI; TUCAN; Tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine
Gene Aliases: CARD8; CARDINAL; DACAR; DAKAR; KIAA0955; NDPP; NDPP1; TUCAN
UniProt ID: (Human) Q9Y2G2
Entrez Gene ID: (Human) 22900
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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