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Invitrogen
CASC5 Polyclonal Antibody
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FIGURE: 1 / 9
CASC5 Antibody (PA5-114943) in ICC/IF
Immunocytochemistry analysis of CASC5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114943) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114943
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KNL1(Accession Q8NG31), corresponding to amino acid residues N1621-V1671.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CASC5.
Target Information
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AF15q14; ALL1-fused gene from chromosome 15q14 protein; Blinkin; blinkin, bub-linking kinetochore protein; Bub-linking kinetochore protein; cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer susceptibility candidate gene 5 protein homolog; Cancer/testis antigen 29; CT29; kinetochore null 1 homolog; Kinetochore scaffold 1; Kinetochore-null protein 1; microcephaly, primary autosomal recessive 4; Outer kinetochore KNL1 complex subunit KNL1; Protein CASC5; Protein D40/AF15q14; protein phosphatase 1, regulatory subunit 55; RAD51 homolog
Gene Aliases: 2310043D08Rik; 5730505K17Rik; AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105; KIAA1570; KNL1; MCPH4; PPP1R55; Rad51; Spc7
UniProt ID: (Mouse) Q66JQ7, (Human) Q8NG31
Entrez Gene ID: (Mouse) 76464, (Rat) 311327, (Human) 57082
acrosome assembly
sister chromatid cohesion
mitotic nuclear division
attachment of spindle microtubules to kinetochore
negative regulation of phosphatase activity
CENP-A containing nucleosome assembly
protein localization to kinetochore
cell division
spindle assembly checkpoint
cell cycle
chromosome segregation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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