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Product Details
PA5-114943
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (PFA fixed) (IHC (PFA)) |
1:50-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:500 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A synthesized peptide derived from human KNL1(Accession Q8NG31), corresponding to amino acid residues E1630-T1659. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2899579 |
Target Information
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AF15q14; ALL1-fused gene from chromosome 15q14 protein; Blinkin; blinkin, bub-linking kinetochore protein; Bub-linking kinetochore protein; cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; kinetochore null 1 homolog; Kinetochore scaffold 1; Kinetochore-null protein 1; microcephaly, primary autosomal recessive 4; Protein CASC5; Protein D40/AF15q14; protein phosphatase 1, regulatory subunit 55; RAD51 homolog
Gene Aliases: 2310043D08Rik; 5730505K17Rik; AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105; KIAA1570; KNL1; MCPH4; PPP1R55; Rad51; Spc7
UniProt ID: (Human) Q8NG31
Entrez Gene ID: (Mouse) 76464, (Rat) 311327, (Human) 57082
acrosome assembly
sister chromatid cohesion
mitotic nuclear division
attachment of spindle microtubules to kinetochore
negative regulation of phosphatase activity
CENP-A containing nucleosome assembly
protein localization to kinetochore
cell division
spindle assembly checkpoint
cell cycle
chromosome segregation
biological_process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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