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Bethyl Laboratories
CASP9/Caspase 9 Polyclonal Antibody
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FIGURE: 1 / 1
CASP9/Caspase 9 Antibody (A305-252A) in WB
Detection of human and mouse CASP9 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CASP9 antibody (Product # A305-252A) (lot A305-252A-1) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-252A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 50 to 100 of human Caspase 9.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP-9; CASP9; caspase 9, apoptosis-related cysteine peptidase; caspase 9b; Caspase-9; Caspase-9 precursor; caspase-9 variant; CASPASE-9c; Caspase9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3; unnamed protein product
Gene Aliases: AI115399; APAF-3; APAF3; AW493809; CASP-9; CASP9; Caspase-9; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211, (Mouse) Q8C3Q9
Entrez Gene ID: (Human) 842, (Mouse) 12371
cysteine-type endopeptidase activity
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
protein kinase binding
identical protein binding
SH3 domain binding
cysteine-type endopeptidase activity involved in apoptotic signaling pathway
protease
response to hypoxia
kidney development
response to ischemia
proteolysis
apoptotic process
cellular response to DNA damage stimulus
intrinsic apoptotic signaling pathway in response to DNA damage
response to UV
protein processing
platelet formation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
cellular response to UV
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
fibroblast apoptotic process
neuron apoptotic process
protein maturation
cellular response to dexamethasone stimulus
response to indole-3-methanol
leukocyte apoptotic process
response to anesthetic
intrinsic apoptotic signaling pathway
positive regulation of execution phase of apoptosis
epithelial cell apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to organic cyclic compound
regulation of response to DNA damage stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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