Invitrogen

CATSPER1 Polyclonal Antibody

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Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

CATSPER1 Antibody (PA5-75788) in WB

Western blot analysis of CATSPER1 in Lane 1: MCF-7 whole cell lysate, Lane 2: sp2/0 whole cell lysate, Lane 3: PC12 whole cell lysate. Samples were incubated with CATSPER1 polyclonal antibody (Product # PA5-75788) at a dilution of 1:500.

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Product Details

PA5-75788

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues in Human CATSPER1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2719516

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion Channels.

International journal of molecular sciences

Baňas Š,Benko F,¿uračka M,Lukáč N,Tvrdá E

PA5-75788 was used in Western Blotting to assess the potential protective abilities of Epicatechin on cryopreserved bovine spermatozoa.

Sat Jan 28 00:00:00 EST 2023

Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion Channels.

International journal of molecular sciences

Baňas Š,Benko F,¿uračka M,Lukáč N,Tvrdá E

PA5-75788 was used in Western Blotting to assess the potential protective abilities of Epicatechin on cryopreserved bovine spermatozoa.

Sat Jan 28 00:00:00 EST 2023

In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A.

International journal of molecular sciences

Benko F,Fialková V,Žiarovská J,¿uračka M,Lukáč N,Tvrdá E

Published figure using CATSPER1 polyclonal antibody (Product # PA5-75788) in Western Blot

Thu Nov 24 00:00:00 EST 2022

In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A.

International journal of molecular sciences

Benko F,Fialková V,Žiarovská J,¿uračka M,Lukáč N,Tvrdá E

Published figure using CATSPER1 polyclonal antibody (Product # PA5-75788) in Western Blot

Thu Nov 24 00:00:00 EST 2022

CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic Semen.

Cells

Manfrevola F,Ferraro B,Sellitto C,Rocco D,Fasano S,Pierantoni R,Chianese R

Published figure using CATSPER1 polyclonal antibody (Product # PA5-75788) in Western Blot

Sat Jul 31 00:00:00 EDT 2021

CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic Semen.

Cells

Manfrevola F,Ferraro B,Sellitto C,Rocco D,Fasano S,Pierantoni R,Chianese R

Published figure using CATSPER1 polyclonal antibody (Product # PA5-75788) in Western Blot

Sat Jul 31 00:00:00 EDT 2021

Low-density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of action.

Molecular reproduction and development

Dalal J,Chandolia RK,Pawaria S,Kumar A,Kumar D,Selokar NL,Andonissamy J,Yadav PS,Kumar P

PA5-75788 was used in Western Blotting to evaluate low-density lipoprotein as a protective agent for sperm during cryopreservation in Murrah buffalo bulls.

Tue Dec 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: cation channel of sperm 1; Cation channel sperm-associated protein 1; cation channel, sperm associated 1; CatSper1; hCatSper; sperm ion channel; sperm protein CATSPER1; sperm-associated cation channel 1

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Gene Aliases: CATSPER; CATSPER1; KSper; SPGF7

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UniProt ID: (Human) Q8NEC5, (Mouse) Q91ZR5

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Entrez Gene ID: (Human) 117144, (Mouse) 225865

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Function(s)

calcium activated cation channel activity voltage-gated calcium channel activity protein binding ion channel activity voltage-gated ion channel activity calcium channel activity ion channel transporter

Process(es)

multicellular organism development spermatogenesis fusion of sperm to egg plasma membrane cell differentiation sperm motility response to progesterone regulation of ion transmembrane transport sperm-egg recognition regulation of calcium ion transport regulation of cilium beat frequency involved in ciliary motility calcium ion transmembrane transport membrane depolarization during action potential transport ion transport calcium ion transport transmembrane transport calcium ion import

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CATSPER1 Polyclonal Antibody

CATSPER1 Antibody (PA5-75788) in WB

Product Details

PA5-75788

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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