PA1-317 detects CBF-beta in human samples.
PA1-317 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates.
The PA1-317 immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta. This sequence is conserved in mouse. PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.
CBF-beta (Core-binding factor beta), also called polyomavirus enhancer binding protein 2 beta (PEBP2B), belongs to the PEBP2/CBF transcription factor family which regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2).
CBF-beta is associated with an inversion of chromosome 16 and is associated with acute myeloid leukemia in humans. CBFbeta forms a heterodimer with RUNX1 (runt-related transcription factor 1), which has a DNA binding domain homologous to the pair-rule protein runt in Drosophila melanogaster. Both RUNX1 and CBFbeta are essential for hematopoiesis. Haploinsufficiency of another runt-related protein, RUNX2 (also called CBFA1), causes cleidocranial dysplasia in humans and is essential in skeletal development by regulating osteoblast differentiation and chondrocyte maturation.
CBF-beta is expressed in all tissues tested. CBF-bet is highly expressed in the thymus, but also abundantly expressed in muscle, lung and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CBF-beta; Core-binding factor subunit beta; PEA2-beta; PEBP2-beta; polyomavirus enhancer binding protein 2, beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit
Gene Aliases: CBFB; PEBP2B
UniProt ID: (Human) Q13951
Entrez Gene ID: (Human) 865