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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA1-317 detects CBF-beta in human samples.
PA1-317 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates.
The PA1-317 immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta. This sequence is conserved in mouse. PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.
CBF-beta (Core-binding factor beta), also called polyomavirus enhancer binding protein 2 beta (PEBP2B), belongs to the PEBP2/CBF transcription factor family which regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2).
CBF-beta is associated with an inversion of chromosome 16 and is associated with acute myeloid leukemia in humans. CBFbeta forms a heterodimer with RUNX1 (runt-related transcription factor 1), which has a DNA binding domain homologous to the pair-rule protein runt in Drosophila melanogaster. Both RUNX1 and CBFbeta are essential for hematopoiesis. Haploinsufficiency of another runt-related protein, RUNX2 (also called CBFA1), causes cleidocranial dysplasia in humans and is essential in skeletal development by regulating osteoblast differentiation and chondrocyte maturation.
CBF-beta is expressed in all tissues tested. CBF-bet is highly expressed in the thymus, but also abundantly expressed in muscle, lung and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CBFβ enhances de novo protein biosynthesis of its binding partners HIV-1 Vif and RUNX1 and potentiates the Vif-induced degradation of APOBEC3G.
PA1-317 was used in western blot to study the mechanisms underlying the effects of CBF-beta on the synthesis of HIV1 Vif and cellular RUNX and on the degradation of APOBEC3G mediated by Vif
|Miyagi E,Kao S,Yedavalli V,Strebel K||Journal of virology (88:4839)||2014|
A stable transcription factor complex nucleated by oligomeric AML1-ETO controls leukaemogenesis.
PA1-317 was used in western blot to study the role of AML1-ETO complex formation in the mechanism by which this transcription factor promotes leukemogenesis
|Sun XJ,Wang Z,Wang L,Jiang Y,Kost N,Soong TD,Chen WY,Tang Z,Nakadai T,Elemento O,Fischle W,Melnick A,Patel DJ,Nimer SD,Roeder RG||Nature (500:93)||2013|
Novel function of the unique N-terminal region of RUNX1c in B cell growth regulation.
PA1-317 was used in western blot to study the role of the N-terminal sequence of RUNX1c in regulating B-cell growth
|Brady G,Elgueta Karstegl C,Farrell PJ||Nucleic acids research (41:1555)||2013|
CBF-beta; PEA2-beta; PEBP2-beta; polyomavirus enhancer binding protein 2, beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit